Precision blending in gradient chromatography -

Precision blending in gradient chromatography

FD sanitary regulator improves blending in gradient chromatography

  • schematic of gradient chromatography skid for sanitary
A “gradient elution skid” or “gradient buffer skid” upstream of the chromatography skid adds buffers at varying ratios and specific timing to perform the gradient chromatography process.

Multiple buffer solutions are stored in on the gradient elution skids and delivered to the chromatography column based on a preset conductivity profile specific to an elution process.

Positive displacement pumps at the outlet of the buffer tanks deliver buffer solutions and blend them to create the desired conductivity profile over time. While the buffer mixture is out of specification as it changes from one setpoint to the next, it is sent to waste until the conductivity reaches the desired profile setpoint.

One method to achieve the proper blend is using an on/off valve with a timer so that the correct amount of buffer solution is added. This method delivers only rough precision. Another method is to use a flow control valve which is more reproducible.

The Equilibar FD sanitary back pressure regulator can be used as a flow control valve, delivering precise blending and instantaneous response to changes in profile setpoint. The immediate response of the FD regulator controls the upstream process quickly, delivering cost savings due to less waste. The Equilibar regulator can maintain consistent flow over a wide range of flow rates, resulting in fine blending and reproducible results for gradient chromatography. 

schematic of gradient chromatography skid

Advantages of the Equilibar design are its small footprint, its high turndown ratio, and excellent response and precision. The electro-pneumatic pilot regulator can be located at the process control system, a distance from the FD valve, for a simple footprint. Equilibar regulators respond instantaneously to changes in setpoint to maintain the desired flow. Because of its lightning fast control capability, the FD regulator has excellent performance in computer automated control processes.

Please contact an Equilibar application engineer to discuss your sanitary process application today.

FD Series Sanitary back pressure regulator designed with ASME BPE in mind. ( product details)

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